PhD Student: Decoding Epigenetic Mechanisms of Gene

About The Role

The group of Fred van Leeuwen is looking for a PhD candidate to join our team working on mechanisms and functions of epigenetic pathways in T cells. In this project, you will study how targeting an epigenetic writer protein called DOT1L reprograms cytotoxic T cells. T cells are crucial players in anti-tumor responses, but tuning their activity remains a challenge. By understanding the epigenetic mechanisms by which DOT1L works, we hope to ultimately strengthen T cells so they can fight cancer cells more effectively. Do you want to join us in uncovering new insights into T-cell biology and help pave the way for more effective cancer immunotherapies? Take this opportunity to grow as a scientist while contributing to research that could make a real difference for cancer patients.

In this research project you will

Apply innovative and complementary strategies in a tractable human T cell model, including CRISPR-Cas9 based genome engineering and epigenetic editing, epigenomics and transcriptomics, and unique DNA-barcode-based epigenetic screens in yeast.

Validate mechanisms in primary human CD8 T cells.

Gain molecular insights to provide leads for improved epigenetic perturbation of CD8 T cells to enhance cytotoxic activity towards cancer cells.

Our group is part of the Division of Gene regulation, which focuses on (epigenetic) mechanisms of genome-organization and gene transcription. We work in close collaboration with the Division of Molecular Oncology and Immunology for complementary expertise on immune cell biology, genetic modification and phenotyping of T cells, and T cell therapy. Our research at the NKI is supported by state-of-the art core facilities, including imaging, flow cytometry, proteomics, genomics, screening, and many more. More information about the Van Leeuwen lab and our recent work on T cells can be found here: www.nki.nl/research/research-groups/fred-van-leeuwen/.

Why the Netherlands Cancer Institute?

Science is at the heart of everything we do at the NKI. But breakthroughs don't happen through science alone; they happen through people – curious minds, bold thinkers, people who challenge assumptions and turn complex questions into meaningful discoveries. Around 600 scientists, together with 150 scientific and 150 administrative professionals, work here across the full spectrum of cancer research, from fundamental science to clinical innovation. With roughly 55 nationalities represented, we form a vibrant international community where English is our shared language and every voice carries equal weight. Our institute sits just 15 minutes by bike from the heart of Amsterdam.